The Production of Indole Acetic Acid from Bacillus thuringiensis and Application in Tissue Culture Technique for Two Cultivars of Solanum tuberosum

Abstract

Bacillus thuringiensis is the most widely used bacterial pesticide, besides its activity against insects; the coming research designed for dual production of delta toxin and phytohormone production the indole acetic acid. A local isolate of B. thuringiensis kurstaki KS3 was earlier isolated and characterized for its activity against insects. The medium minimal salt (MS) was used for the production of IAA. IAA activity that evaluated in tissue culture technique for two varieties  cultivars of Solanum tuberosum L and the results indicated that phytohormone IAA extract gave a positive response for the varieties cultivars Rivera and Bureen, that   tissue cultures.Statistical analysis revealed no significant differences between the two varieties  cultivars about different concentrations of extracted IAA and laboratory-prepared IAA and the negative control in response to plant high, branch number and length, and leaves number. Whereas, LSD showed that Rivira was superior in root number and length.