Isolation and Diagnoses Escherichia coli from Patients at Hospital in Baghdad

Abstract

The study included collecting (100) pathological bacterial samples from patients with urinary tract infections, bacteremia, and others from Yarmouk Teaching Hospital and Medical City hospitals. Escherichia coli isolates were diagnosed using morphological, microscopic, and biochemical methods. Tests of locally isolated bacterial samples proved that all isolates used in laboratory experiments were Escherichia coli. The sensitivity and resistance of the identified bacterial isolates to 10 different antibiotics were tested using the disc diffusion method. The results showed that all bacterial isolates were sensitive to the antibiotic Ciprofloxacin, which gave the highest rate of inhibition diameter for E. coli compared to other bacterial isolates through sensitivity testing. A significant increase in the diameter of inhibition was observed for the gold nanoparticles concentration (4.0 mg/ml), which recorded the highest average diameter of inhibition (11.6 mg/ml) compared to the other concentrations of the mixed extract, the aqueous extract, distilled water, and the standard antibiotic. The results of the experiment with three replicates also showed that all isolates had the ability to form biofilms, but to varying degrees under the same experimental conditions.