Effect of Concentrations of Growth Regulator Thidiazuron 2,4-D and Various Interventions on Induction and Growth of Callus from Leaves of Catharanthus roseus (L.) G.Don.

Abstract

In this study, the results show the effect of the concentrations of the growth regulators Thidiazuron (TDZ) and 2,4-D on the percentage of callus induction from parts of the leaf of the Ayna plant. The statistical analysis showed that there are significant differences between the concentrations of the two growth regulators and their interactions to induce callus. The concentration of 3 mg.L-¹ of 2,4-D was the best rate of callus induction, while the concentration of 1.5 mg.L-¹ of TDZ excelled with an induction rate of up to 100%. While the comparison sample did not give any callus induction.